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epsC [2018-11-11 15:59:23]
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epsC [2018-11-11 15:59:23]

UDP-N-acetylglucosamine 4,6-dehydratase, required for extracellular polysaccharide synthesis, this gene is inactive in B. subtilis 168
locus
BSU34350
pI
8.77
mw
66.09 kDa
protein length
598 aa Sequence Blast
gene length
1794 bp Sequence Blast
function
[category|SW 4.1.2|Biofilm formation], biosynthesis of N,N'-diacetylbacillosamine
product
UDP-N-acetylglucosamine 4,6-dehydratase
essential
no
synonyms
yveM
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.1|Matrix polysaccharide synthesis]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,526,407 → 3,528,203

    The protein

    Protein family

  • polysaccharide synthase family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|F42B27D5D7F3F39828940E377ABCC0730B25EA88|YodU]:
  • [protein|240CD6EA3793821F5109252BDEF69C0120E454EF|YpqP]:

    • [SW|Cofactors]

  • NAD+ [pubmed|30222950]

    • [SW|Localization]

  • cell membrane (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|F6C4634BF871D9A01E27014FFED7527C54BF560D|epsA]-[gene|9B668909E1380B287ACE58561DCF45FC29184D8B|epsB]-[gene|5DB168A3D087AAEB003D7548A1A4356ABD07F712|epsC]-[gene|223E979276F07D69D0DDAEE906023A8AB4F37F8E|epsD]-[gene|5D717F9F54693FD0AA8BC49E10348637858F88B9|epsE]-[gene|DB47BA2A5E3BED51DD8630E7D321C099D74B46CF|epsF]-[gene|C4D81976AAE888C4FE89A2EE5CF9A763CAD645FF|epsG]-[gene|BD843389F95BFB905071547AB243413A617F12A8|epsH]-[gene|6EBD8F7065B6C1996067F3120DD9CFEA91D3A444|epsI]-[gene|5272E807B6B98541EA155AA9748320E25767F096|epsJ]-[gene|66076952E512D53FC1FC62455A86ACFA21AE120D|epsK]-[gene|D67665412B78B56752B460219445E4BEF52C3A1A|epsL]-[gene|40EA584CF77FFF7D7E4E74B414EC69ADE3A1585B|epsM]-[gene|0D24AB8D446CFFBC6DEAD3C10D1FA59551545FE8|epsN]-[gene|52A47B49B1FB955EE7E3C316B57A2B4134F78480|epsO]
    description
    [Pubmed|15661000]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|15661000], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA]: activation, [Pubmed|23646920], in [regulon|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]: anti-activation, (prevents binding of [protein|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA]) [Pubmed|23646920], in [regulon|5A6FBAE6553343092862CB79E150F934978C32A9|SinR regulon]
  • [regulon|EAR riboswitch|EAR riboswitch]: processive antitermination, in [regulon|EAR riboswitch|EAR riboswitch]

    • regulation

  • repressed by [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR] [Pubmed|15661000]

    • additional information

  • induction by sequestration of [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR] by [protein|24D6A7DDCB25B99FBB670E9203F826596916B35C|SinI] or [protein|C17C296F3EB2E106C380E3B5D784F3FA63F4C9B7|SlrA] [PubMed|15661000,19788541]
  • expression is increased in [gene|21D260E900776EAAF4B6000A3365DCA9241EACA6|rsiX] mutants [pubmed|32483306]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A071 (yveM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/71 NBRP B. subtilis, Japan]
  • BKE34350 (Δ[gene|5DB168A3D087AAEB003D7548A1A4356ABD07F712|epsC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGACAGTCTTCTCCGGT, downstream forward: _UP4_AGCGTTCATTAGGGGGAGTG
  • BKK34350 (Δ[gene|5DB168A3D087AAEB003D7548A1A4356ABD07F712|epsC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGACAGTCTTCTCCGGT, downstream forward: _UP4_AGCGTTCATTAGGGGGAGTG

    • labs

  • [SW|Richard Losick], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]

    • References

    Reviews

  • 20384681,20735481

    • Research papers

  • 27655338,30222950

    • The EAR [SW|RNA switch]

  • 20374491,20230605